A comparative study of HIV-1 clade C \u3ci\u3eenv\u3c/i\u3e evolution in a Zambian infant with an infected rhesus macaque during disease progression

Objective—To evaluate whether HIV-1 clade C (HIV-C) envelope variations that arise during disease progression in rhesus macaque model reflect changes that occur naturally in human infection. Design—An infant macaque was infected with SHIV-1157i, an R5 tropic clade C SHIV (SHIV-C) which expresses a primary HIV-C envelope derived from an infected human infant, and monitored over a five-year period. Genetic variation of the V1-V5 envelope region, which is the main target for humoral immune responses, derived from the infected macaque and infant was examined. Methods—V1-V5 envelope region were cloned and sequenced from longitudinal PBMC samples collected from the infected macaque and infant. Phylogenetic analysis (phylogenetic tree, diversity, divergence, ratio of non-synonymous (dN) and synonymous substitution (dS) and dN distribution) were performed. Plasma RNA viral load, CD4+ T-cell count, changes in the length of V1-V5 region, putative N-linked glycosylation sites (PNGSs) number and distribution were also measured. Results—Phylogenetic analysis revealed that changes in the macaque closely reflected those of infant during disease progression. Similar distribution patterns of dN and hot spots were observed between the macaque and infant. Analysis of PNGSs revealed several common variations between the virus populations in the two host species. These variations correlate with decline of CD4 count in the macaque and might be linked with disease progression. Conclusion—SHIV-C infection of macaque is a relevant animal model for studying variation of primary HIV-C envelope during disease progression and could be used to analyze the selection pressures that are associated with those changes