Response of hydrolytic enzyme activities and nitrogen mineralization to fertilizer and organic matter application in subtropical paddy soils

Drivers of nitrogen (N) mineralization in paddy soils, especially under anaerobic soil conditions, are elusive. The influences of exogenous organic matter (OM) and fertilizer application on the activities of five relevant enzymes (β-glucosaminidase, β-glucosidase, L-glutaminase, urease and arylamidase) were measured in two long-term field experiments. Of the two field experiments, the 18-year field experiment was established in a weathered terrace soil with rice-wheat crop rotation at the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU) farm with five OM treatments and two levels of mineral N fertilizer. The 30-year experiment was established in a young floodplain soil with rice-rice crop rotation at the Bangladesh Agricultural University (BAU) farm with five mineral fertilizer treatments including one with farm yard manure. At BSMRAU, N fertilizer and OM amendments significantly increased all enzyme activities, suggesting the availability of primarily substrate for microbial activity. Whereas at BAU, non-responsiveness of β-glucosidase activity, suggesting that fertilizer and OM amendments had little effect on overall soil microbial activity. Nevertheless the microbial demand for N, β-glucosaminidase and L-glutaminase activities differed among the treatments (P