Population dynamics in mixed cultures of Neochloris Oleoabundans and native microalgae from water of a polluted river and isolation of a diatom consortium for the production of lipid rich biomass

The production of biodiesel utilizing microalgae has driven innovation worldwide, especially trying to overcome the current economic and technological limitations of the whole process. Within these efforts, the use of wastewater to cultivate oleaginous microalgae or the use of dual-purpose microalgae-bacteria-based systems that treat wastewater and produce oleaginous microalgae have become an attractive alternative. The aim of this work was to evaluate the population dynamics which occurred in mixed cultures of Neochloris oleoabundans with other native microalgae, in mixtures of a synthetic medium (BBM) and water of an urban polluted river. The effect of temperature, nutrient availability and the microscopic monitoring of the population dynamics in such mixed cultures were carried out. Furthermore, the isolation of the predominant consortium of diatoms and the evaluation of its kinetics of growth and its capacity for removal of pollutants was also performed. Results indicated that such green microalgae only predominated in mixtures containing 80% or 60% of the synthetic medium. In mixtures containing a volume of the polluted river higher than 40%, other microalgae predominated, especially diatoms of various genera. The diatom consortium isolated from a 100% of the river's water sampled in spring (April), was formed mainly by a population of Nitzchia frustulum and in less extent of Navicula sp. It showed a significantly higher specific growth rate when cultivated in water from the river, compared to cultures in synthetic modified diatom medium (MDM) and at 32 degrees C, compared to cultures incubated at 25 degrees C. The consortium was able to remove 95.45% and 95.78% of ammonia nitrogen, 60% and 62.5% of nitrates at 32 degrees C and 25 degrees C, respectively, after 2 days. It also removed 95% of phosphates at 32 degrees C and 67% at 25 degrees C after 4 days from the polluted river. Diatoms also showed significant accumulation of lipids after 10 days of cultivation when stained with Sudan III. In conclusion, such diatom consortium showed a large potential for being used in a dual-purpose system that could treat the water from polluted streams and that could produce lipid rich biomass