Developments in detection and determination of aflatoxins

Since the discovery of aflatoxins in the 1960s, much research has focused on detecting the toxins in contaminated food and feedstuffs in the interest of public safety. Most traditional detection methods involved lengthy culturing and/or separation techniques or analytical instrumentation and complex, multistep procedures that required destruction of samples for accurate toxin determination. With more regulations for acceptable levels of aflatoxins in place, modern analytical methods have become quite sophisticated, capable of achieving results with very high precision and accuracy, suitable for regulatory laboratories and for post-harvest sample testing in developed countries. Unfortunately, many countries around the world that are affected by the aflatoxin problem do not have ready access to high performance liquid chromatography and mass spectrometry instrumentation and require alternate, readily available and simple detection methods that may be used by small holdings farmers in developing countries. This paper presents an overview of the existing detection and/or determination methods for aflatoxins. The traditional, quantitative, chemically-based analytical strategies for detecting aflatoxins in maize and their evolution to the modern instrumentation routinely used in developed countries are reviewed. Additionally, novel, more streamlined, user-friendly and in some instances, non-destructive, methods that may be useful for semi-quantitative or qualitative, quick-screening of contaminated maize samples appropriate also for use in developing countries, are discussed