Microfluidics can be used to generate flow-driven gradients of chemotropic guidance cues with precisely controlled steepnesses for indefinite lengths of time. Neuronal cells grown in the presence of these gradients can be studied for their response to the effects exerted by the cues. Here we describe a polydimethylsiloxane (PDMS) microfluidics chamber capable of producing linear gradients of soluble factors, stable for at least 18 h, suitable for axon guidance studies. Using this device we demonstrate turning of superior cervical ganglion axons by gradients of nerve growth factor (NGF). The chamber produces robust gradients, is inexpensive to mass produce, can be mounted on a tissue culture dish or glass coverslip for long term time-lapse m...
A microfluidic network (microFN) etched into a silicon wafer was used to deliver protein solutions c...
AbstractThe extracellular availability of growth factors, hormones, chemokines, and neurotransmitter...
Axonal guidance is a very important process during brain development, allowing to give it its struct...
A user-friendly microfluidic chamber produces a passive, stable gradient of soluble cues for imaging...
Over the past decades, various microfluidic devices have been developed to investigate the role of t...
In the injured adult nervous system, re-establishment of growth-promoting molecular gradients is kno...
During nervous system development growing axons are often guided by diffusible chemical gradients. A...
Thesis (M. Eng.)--Massachusetts Institute of Technology, Biological Engineering Division, 2007.Inclu...
During development a variety of cell types are guided by molecular concentration gradients to form t...
During development a variety of cell types are guided by molecular concentration gradients to form t...
Biochemical and physical guidance cues are both pivotal for axonal guidance and nerve regeneration. ...
Axonal outgrowth and guidance play an important role in wiring the developing and regenerating nervo...
We have developed a cast microfluidic chip for concentration gradient generation that contains a thi...
Enhancing and directing axon regeneration through an injury site represents a prime goal for therapi...
Axonal dysfunction and degeneration are important pathological features of central nervous system (C...
A microfluidic network (microFN) etched into a silicon wafer was used to deliver protein solutions c...
AbstractThe extracellular availability of growth factors, hormones, chemokines, and neurotransmitter...
Axonal guidance is a very important process during brain development, allowing to give it its struct...
A user-friendly microfluidic chamber produces a passive, stable gradient of soluble cues for imaging...
Over the past decades, various microfluidic devices have been developed to investigate the role of t...
In the injured adult nervous system, re-establishment of growth-promoting molecular gradients is kno...
During nervous system development growing axons are often guided by diffusible chemical gradients. A...
Thesis (M. Eng.)--Massachusetts Institute of Technology, Biological Engineering Division, 2007.Inclu...
During development a variety of cell types are guided by molecular concentration gradients to form t...
During development a variety of cell types are guided by molecular concentration gradients to form t...
Biochemical and physical guidance cues are both pivotal for axonal guidance and nerve regeneration. ...
Axonal outgrowth and guidance play an important role in wiring the developing and regenerating nervo...
We have developed a cast microfluidic chip for concentration gradient generation that contains a thi...
Enhancing and directing axon regeneration through an injury site represents a prime goal for therapi...
Axonal dysfunction and degeneration are important pathological features of central nervous system (C...
A microfluidic network (microFN) etched into a silicon wafer was used to deliver protein solutions c...
AbstractThe extracellular availability of growth factors, hormones, chemokines, and neurotransmitter...
Axonal guidance is a very important process during brain development, allowing to give it its struct...