Raman spectroscopic and potentiometric studies of acidity level and dissociation of citric acid in aqueous solution

NoThe dissociation constant is one of the most important characteristics of a pharmaceutical chemical moiety which has to be estimated with accuracy. The development of in-situ speciation methods in solutions with parallel measurements using Raman spectroscopy (molecular) and pH (macroscopic) for the identification, characterization, and quantitative determination of citric acid species in aqueous solution by numerical data treatment using a multiwavelength curve fitting program over a range of pH values is described. As a result, the first, second and third stepwise dissociation constants of citric acid have been evaluated as 3.02±0.06, 4.78±0.06 and 6.02±0.04, respectively. From these data over the pH range 2.38-6.16 an excellent agreement with literature values was achieved