The chloroplast small heat shock protein undergoes oxidation-dependent conformational changes and may protect plants from oxidative stress
- Härndahl, Ulrika
- Buffoni Hall, Roberta
- Osteryoung, K.W.
- Vierling, E.
- Bornman, Janet
- Sundby, Cecilia
DOIAbstract
The nuclear-encoded chloroplast-localized Hsp21 is an oligomeric heat shock protein (Hsp), belonging to the protein family of small Hsps and @a-crystallins. We have investigated the effects of high temperature and oxidation treatments on the structural properties of Hsp21, both in purified recombinant form and in transgenicArabidopsis thalianaplants engineered to constitutively overexpress Hsp21. A conformational change was observed for the 300 kDa oligomeric Hsp21 protein during moderate heat stress (=<40oC) ofArabidopsisplants, as judged by a shift to lower mobility in non-denaturing electrophoresis. Similar changes in mobility were observed when purified recombinant Hsp21 protein was subjected to an oxidant. Exposure of Hsp21 protein to ...
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