Sampling and Selective Detection of Peptides and Proteins Emphazing Microdialysis,Bio-recognition Assays and MALDI-TOF MS

The use of microdialysis sampling and flow immunochemical detection as well as MALDI-TOF MS for peptide and protein analysis is described. Sampling of peptides and smaller proteins was investigated with different membranes. Cyclodextrines additives and an on-line push-pull microdialysis sampling method was used with the result of a higher sampling efficiency compared to conventional microdialysis sampling. Real-time flow immuno assays systems were developed for the quantification of Interleukin-8 and Interleukin10 in cell samples. Optimisation with regards to affinity support selection as well as carrier buffer composition were performed. Connection of capillary LC with MALDI-TOF MS was made utilising an m-dispenser technique enabling sample handling of picoliter volumes. A novel sample preparation technique for the uniform coating of MALDI targets was developed