Solute transport on the sub 100 ms scale across the lipid bilayer membrane of individual proteoliposomes
- Ohlsson, G.
- Tabaei, S. R.
- Beech, Jason
- Kvassman, Jan
- Johanson, Urban
- Kjellbom, Per
- Tegenfeldt, Jonas
- Höök, Fredrik
DOIAbstract
Screening assays designed to probe ligand and drug-candidate regulation of membrane proteins responsible for ion-translocation across the cell membrane are wide spread, while efficient means to screen membrane-protein facilitated transport of uncharged solutes are sparse. We report on a microfluidic-based system to monitor transport of uncharged solutes across the membrane of multiple (>100) individually resolved surface-immobilized liposomes. This was accomplished by rapidly switching (<10 ms) the solution above dye-containing liposomes immobilized on the floor of a microfluidic channel. With liposomes encapsulating the pH-sensitive dye carboxyfluorescein (CF), internal changes in pH induced by transport of a weak acid (acetic acid) could ...
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