Magneto-Optical Measurements of the Pigments in Fully Active Photosystem II Core Complexes from Plants
- Smith, Paul J
- Peterson Årsköld, Sindra
- Masters, Vanessa M
- Wydrzynski, Tom
- Styring, Stenbjörn
- Krausz, Elmars
- Pace, Ron J
DOIAbstract
Preparation of a minimum PSII core complex from spinach is described, containing four Mn per reaction center (RC) and exhibiting high O2 evolving activity [~4000 mol of O2 (mg of chl)-1 h-1]. The complex consists of the CP47 and CP43 chlorophyll binding proteins, the RC D1/D2 pair, the cytochrome b559 subunits, and the Mn-stabilizing psbO (33 kDa) protein, all present in the same stoichiometric amounts found in the parent PSII membranes. Several small subunits are also present. The cyt b559 content is 1.0 per RC in core complexes and PSII membranes. The total chlorophyll content is 32 chl a and <1 chl b per RC, the lowest yet reported for any active PSII preparation. The core complex exhibits the characteristic EPR signals seen in the S2 st...
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