Development of an embryotoxicity test for Enchytraeus crypticus: the effect of Cd
- Gonçalves, Micael F. M.
- Bicho, Rita C.
- Rêma, Alexandra
- Soares, Amadeu M. V. M.
- Faustino, Augusto M. R.
- Amorim, Mónica J. B.
DOI Publisher
Elsevier BV ISSN
0045-6535 Journal
Chemosphere Citation count (estimate)
5Abstract
The existing standard enchytraeid reproduction test (ERT) concerns the assessment of effects on survival and reproduction. In the present study we optimized and propose an embryotoxicity test using Enchytraeus crypticus. Cadmium (Cd) was used as a test substance. Endpoints evaluated were embryo development, number of embryonic structures, Calcium (Ca) channels quantification and hatching success with macroscopic monitoring, histological and immunohistochemistry analysis. Results showed that Cd is embryotoxic for this species, causing a decrease in the hatching success (EC50 = 3.1 mg/kg), a delay or disruption in formation of embryonic structures depending on concentrations (<5 mg Cd/kg or P16 mg Cd/kg). Results from immunohistochemis...
Add to ORKG