Phagocytic NADPH oxidase-dependent superoxide production stimulates matrix metalloproteinase-9: implications for human atherosclerosis
- Zalba, G. (Guillermo)
- Fortuño, A. (Ana)
- Orbe, J. (Josune)
- San-Jose, G. (Gorka)
- Moreno, M.U. (María Ujué)
- Belzunce, M. (Miriam)
- Rodriguez, J.A. (José Antonio)
- Beloqui, O. (Óscar)
- Paramo, J.A. (José Antonio)
- Diez-Martinez, J. (Javier)
Publication date
January 2007
DOI Publisher
Ovid Technologies (Wolters Kluwer Health) ISSN
1079-5642Abstract
OBJECTIVE: Data suggest that matrix metalloproteinase-9 (MMP-9) has a role in atherosclerosis. The phagocytic NADPH oxidase has been also associated with atherosclerosis. This study aimed to investigate the association between phagocytic NADPH oxidase and MMP-9 in human atherosclerosis. METHODS AND RESULTS: In vitro experiments performed in human monocytes showed that NADPH oxidase activation enhanced MMP-9 secretion and activity, determined by enzyme-linked immunosorbent assay and zymography, respectively. Immunohistochemical study showed that phagocytic NADPH oxidase localized with MMP-9 in endarterectomies from patients with carotid stenosis. In addition, a positive relationship (P<0.001) was found between phagocytic NADPH oxidase...
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