Background/Aim: Epithelial-mesenchymal communication plays a key role in tissue homeostasis and abnormal signaling contributes to chronic airways disease such as COPD. Most in vitro models are limited in complexity and poorly represent this epithelial-mesenchymal trophic unit. We postulated that cellular outgrowth from bronchial tissue would enable development of a mucosal structure that recapitulates better in vivo tissue architecture. Materials and Methods: Bronchial tissue was embedded in Matrigel and outgrowth cultures monitored using time-lapse microscopy, electrical resistance, light and electron microscopy. Cultures were challenged repetitively with cigarette smoke extract (CSE). Results: The outgrowths formed as a multicellular shee...
The airway epithelium is exposed to a variety of harmful agents during breathing and appro-priate ce...
Air-liquid interface (ALI) cultures are frequently used in lung research but require substantial cel...
peer reviewedThe discovery and evaluation of new active drugs will be easier thanks to the developme...
Abstract BACKGROUND/AIM: Epithelial-mesenchymal communication plays a key role in tissue homeostasi...
Recently it has been hypothesized that a reactivation of the epithelial-mesenchymal trophic unit (EM...
Rationale: A key factor involved with the progressive decline in lung function in severeasthma is ai...
Summary: Complex human airway cellular organization where extracellular matrix (ECM) and epithelial ...
The human respiratory muco-ciliary epithelium consists of different cell types, connected by tight j...
In vitro airway models are increasingly important for pathomechanistic analyses of respiratory disea...
Abstract Background The collagen gel contraction assay measures gel size to assess the contraction o...
Cigarette smoke (CS) and air pollutants (AP) activate pathological processes in bronchial epithelial...
Complex human airway cellular organisation where extracellular matrix (ECM), epithelial and stromal ...
As the lung develops, epithelial-mesenchymal crosstalk is essential for the developmental processes ...
Introduction: ex vivo airway epithelial cell models provide a biologically representative platform f...
© 2021 Qianyu ChenRespiratory diseases, including severe asthma and chronic obstructive pulmonary di...
The airway epithelium is exposed to a variety of harmful agents during breathing and appro-priate ce...
Air-liquid interface (ALI) cultures are frequently used in lung research but require substantial cel...
peer reviewedThe discovery and evaluation of new active drugs will be easier thanks to the developme...
Abstract BACKGROUND/AIM: Epithelial-mesenchymal communication plays a key role in tissue homeostasi...
Recently it has been hypothesized that a reactivation of the epithelial-mesenchymal trophic unit (EM...
Rationale: A key factor involved with the progressive decline in lung function in severeasthma is ai...
Summary: Complex human airway cellular organization where extracellular matrix (ECM) and epithelial ...
The human respiratory muco-ciliary epithelium consists of different cell types, connected by tight j...
In vitro airway models are increasingly important for pathomechanistic analyses of respiratory disea...
Abstract Background The collagen gel contraction assay measures gel size to assess the contraction o...
Cigarette smoke (CS) and air pollutants (AP) activate pathological processes in bronchial epithelial...
Complex human airway cellular organisation where extracellular matrix (ECM), epithelial and stromal ...
As the lung develops, epithelial-mesenchymal crosstalk is essential for the developmental processes ...
Introduction: ex vivo airway epithelial cell models provide a biologically representative platform f...
© 2021 Qianyu ChenRespiratory diseases, including severe asthma and chronic obstructive pulmonary di...
The airway epithelium is exposed to a variety of harmful agents during breathing and appro-priate ce...
Air-liquid interface (ALI) cultures are frequently used in lung research but require substantial cel...
peer reviewedThe discovery and evaluation of new active drugs will be easier thanks to the developme...