The primary organization of nucleosomal core particles from actively dividing cells of lily

AbstractNucleosomal core particles containing different variants of histones H2A and H2B were isolated from actively growing sepals of lily buds (Lilium candida, L.). The particles had decreased electrophoretic mobility relative to those from other sources. Comparison of the arrangement of histones along DNA in lily nucleosomes with previously obtained data for nucleosomes from yeast and animal cells, either active or repressed with regard to transcription and replication, has revealed no significant differences. The results suggest that the structure of nucleosomal core particles is highly conserved in all eukaryotes, whatever the functional state of the cell and the structure of histones H2A and H2B