Anti-Nociceptive, Analgesic and Pathohistological Effects of Intrathecal Dexmedetomidine and Bupivacaine in Rats

AbstractBackground and ObjectivesThis study investigates analgesic and nociceptive effects of adding dexmedetomidine to bupivacaine neuraxial anesthesia through Tail-flick (TF) and Hot-plate (HP) tests and the pathohistological changes on spinal nerves and nerve roots through light microscopy.MethodsForty anesthetized, male Sprague-Dawley rats were intrathecally catheterized. Basal values of TF and HP tests were measured before and after catheterization. Thirty-six successfully catheterized rats were assigned to four groups. Group B received 10μg bupivacaine, Group BD3 received 10μg bupivacaine + 3μg dexmedetomidine, Group BD10 received 10μg bupivacaine + 10μg dexmedetomidine and Control group received 10μL volume of artificial cerebrospinal fluid. TF and HP tests were performed between the 5th and 300th minutes of drug administration. Twenty-four hours after administration of drugs, rats were sacrificed and spinal cord and nerve roots were removed for pathological investigation.ResultsBaseline values of the TF and HP tests were not statistically different among the groups (6.8 ± 0.15 s). TF and HP latencies in the Control group did not change significantly during the study. TF and HP test results showed that adding 3 and 10μg dexmedetomidine caused a dose- dependent increase in duration and amplitude of analgesic and nociceptive effect of bupivacaine (TF: 37.52 ± 1.08%, 57.86 ± 1.16% respectively, HP: 44.24 ± 1.15%, 68.43 ± 1.24% respectively).ConclusionsThere were no apparent pathohistological changes at least 24hours after the intrathecal administration of a single dose of dexmedetomidine 3μg and 10μg. Dexmedetomidine added to bupivacaine for spinal block improves analgesia and prolongs block duration