Activation of mouse phosphodiesterase 3B gene promoter by adipocyte differentiation in 3T3-L1 cells
- Niiya, Toshiyuki
- Osawa, Haruhiko
- Onuma, Hiroshi
- Suzuki, Yoshifumi
- Taira, Masato
- Yamada, Kazuya
- Makino, Hideichi
Publication date
September 2001
DOI Publisher
Federation of European Biochemical Societies. Published by Elsevier B.V. ISSN
0014-5793Abstract
AbstractActivation of phosphodiesterase (PDE) 3B reduces free fatty acid output from adipocytes. Induction of PDE3B gene expression by adipocyte differentiation could improve insulin resistance. To examine whether the PDE3B promoter is activated by this differentiation, the 5′ flanking sequence of the mouse PDE3B gene was isolated. The transcription initiation site was determined to be located 195 bp upstream of the translation start site. No putative binding site for peroxisome proliferator-activated receptor γ was found within 2 kb upstream of the transcription initiation site. This region had promoter activity, which was further activated on adipocyte differentiation in 3T3-L1 cells
Add to ORKG