Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid
- Dautigny, André
- Alliel, Patrick M.
- d'Auriol, Luc
- Dinh, Daniéle Pham
- Nussbaum, Jean-Louis
- Galibert, Francis
- Joliès, Pierre
Publication date
August 1985
DOI Publisher
Published by Elsevier B.V. ISSN
0014-5793 Citation count (estimate)
40Abstract
AbstractA cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues
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