Exploring infrared wavelength matrix-assisted laser desorption/ionization of proteins with delayed-extraction time-of-flight mass spectrometry

Zhang, Wenzhu
Niu, Shufang
Chait, Brian T.

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Publication date

September 1998

DOI

10.1016/S1044-0305(98)00060-9

Publisher

American Society for Mass Spectrometry. Published by Elsevier B.V.

ISSN

1044-0305

Abstract

AbstractWe report a study of the application of delayed extraction (DE) to infrared-wavelength matrix-assisted time-of-flight mass spectrometry (IR-MALDI-TOF-MS) of proteins. The shapes of the spectral peaks obtained with DE-IR-MALDI-MS are compared with those obtained from the same samples and matrix using continuous extraction (CE) IR-MALDI-MS. Application of DE results in significant improvements in the peak resolution, revealing spectral features (in proteins with molecular masses <12 kDa) that were not resolved in the corresponding CE-IR-MALDI mass spectra. Particularly significant is a series of peaks on the high mass side of the protonated protein peaks that arise through replacement of protons by adventitious sodium ions in the samp...