AbstractSmall plus-stranded RNA viruses do not code for RNA helicases that would facilitate the proper folding of viral RNAs during replication. Instead, these viruses might use RNA chaperones as shown here for the essential p33 replication protein of Tomato bushy stunt virus (TBSV). In vitro experiments demonstrate that the purified recombinant p33 promotes strand separation of a DNA/RNA duplex. In addition, p33 renders dsRNA templates sensitive to single-strand specific S1 nuclease, suggesting that p33 can destabilize highly structured RNAs. We also demonstrate that the RNA chaperone activity of p33 facilitates self-cleavage by a ribozyme in vitro. In addition, purified p33 facilitates in vitro RNA synthesis on double-stranded (ds)RNA tem...
Reoviruses are important human, animal and plant pathogens having 10-12 segments of double-stranded ...
AbstractThe assembly of the functional replicase complex via protein:protein and RNA:protein interac...
Replication of genetic material is the most important and central process during the viral life cycl...
AbstractSmall plus-stranded RNA viruses do not code for RNA helicases that would facilitate the prop...
Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) req...
Replication of plus-strand RNA viruses depends on recruited host factors that aid several critical s...
Tombusviruses are single, positive strand RNA viruses of plants, often associated with parasitic def...
RNA molecules are functionally diverse in part due to their extreme structural flexibility that allo...
AbstractTomato bushy stunt virus (TBSV), a tombusvirus with a non-segmented, plus-stranded RNA genom...
AbstractRecruited host factors aid replication of plus-strand RNA viruses. In this paper, we show th...
Replication of plus-strand RNA viruses depends on recruited host factors that aid several critical s...
AbstractMany plus-strand (+)RNA viruses co-opt protein chaperones from the host cell to assist the s...
AbstractMaintenance of genome integrity is of major importance for plus-stranded RNA viruses that ar...
AbstractAn ideal system to investigate individual determinants of the replication process of (+)-str...
AbstractPlus-stranded RNA viruses replicate efficiently in infected hosts producing numerous copies ...
Reoviruses are important human, animal and plant pathogens having 10-12 segments of double-stranded ...
AbstractThe assembly of the functional replicase complex via protein:protein and RNA:protein interac...
Replication of genetic material is the most important and central process during the viral life cycl...
AbstractSmall plus-stranded RNA viruses do not code for RNA helicases that would facilitate the prop...
Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) req...
Replication of plus-strand RNA viruses depends on recruited host factors that aid several critical s...
Tombusviruses are single, positive strand RNA viruses of plants, often associated with parasitic def...
RNA molecules are functionally diverse in part due to their extreme structural flexibility that allo...
AbstractTomato bushy stunt virus (TBSV), a tombusvirus with a non-segmented, plus-stranded RNA genom...
AbstractRecruited host factors aid replication of plus-strand RNA viruses. In this paper, we show th...
Replication of plus-strand RNA viruses depends on recruited host factors that aid several critical s...
AbstractMany plus-strand (+)RNA viruses co-opt protein chaperones from the host cell to assist the s...
AbstractMaintenance of genome integrity is of major importance for plus-stranded RNA viruses that ar...
AbstractAn ideal system to investigate individual determinants of the replication process of (+)-str...
AbstractPlus-stranded RNA viruses replicate efficiently in infected hosts producing numerous copies ...
Reoviruses are important human, animal and plant pathogens having 10-12 segments of double-stranded ...
AbstractThe assembly of the functional replicase complex via protein:protein and RNA:protein interac...
Replication of genetic material is the most important and central process during the viral life cycl...