Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast
- Centeno, Francisco
- Deschamps, Stéphane
- Lompré, Anne-Marie
- Anger, Marielle
- Moutin, Marie-Jo
- Dupont, Yves
- Palmgren, Michael Gjedde
- Villalba, Jose Manuel
- Møller, Jesper Vuust
- Falson, Pierre
- le Maire, Marc
DOIAbstract
AbstractWe describe here an easy system for the production of mg amounts of the rabbit Ca2+-ATPase SERCA 1a in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca2+-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles
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