GTPγS-induced solubilization of actin and myosin from rabbit peritoneal neutrophil membrane

AbstractAddition of guanosine 5'-(3-O-thio)triphosphate (GTPγS) to the membrane fraction isolated from rabbit peritoneal neutrophils results in the solubilization of several proteins from the membrane. The major proteins are of 180 kDa (myosin) and 43 kDa (actin). The effect is observed with a half-maximum GTPγS concentration of 70 μM. The potencies of various nucleotides are compared: GTPγS ⪢ GtP ATP ⪢ GDP, GMP, cGMP, cAMP. The effect does not require calcium and is not inhibited by using membranes perpared from cells that have been pretreated with pertussis toxin