Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: an evaluation of four procedures
- Berg, H.F.
- Maraha, B.
- Bergmans, A.M.C.
- Zee, A. van der
- Kluytmans, J.A.J.W.
- Peeters, M.F.
Publication date
February 2003
DOI Publisher
European Society of Clinical Infectious Diseases. Published by Elsevier Ltd. ISSN
1198-743X Citation count (estimate)
10Abstract
ABSTRACTThe objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer—saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion—forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro
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