Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract

AbstractThis study investigated the phytochemical, antioxidative, antimicrobial and cytotoxic effects of Leea indica leaf ethanol extract. Phytochemical values namely total phenolic and flavonoid contents, total antioxidant capacity, DPPH radical scavenging effect, FeCl3 reducing power, DMSO superoxide scavenging effect and Iron chelating effects were studied by established methods. Antibacterial, antifungal and cytotoxic effects were screened by disk diffusion technique, food poison technique and brine shrimp bioassay, respectively. Results showed the total phenolic content 24.00±0.81g GAE/100g, total flavonoid content 194.68±2.43g quercetin/100g and total antioxidant capacity 106.61±1.84g AA/100g dry extract. Significant (P<0.05) IC50 values compared to respective standards were recorded in DPPH radical scavenging (139.83±1.40μg/ml), FeCl3 reduction (16.48±0.64μg/ml), DMSO superoxide scavenging (676.08±5.80μg/ml) and Iron chelating (519.33±16.96μg/ml) methods. In antibacterial screening, the extract showed significant (P