The preparation and properties of novel reversible polymer-protein conjugates 2-ω-Methoxypolyethylene (5000) glycoxymethylene-3-methylmaleyl conjugates of plasminogen activators

AbstractThe preparation of a reagent capable of reversibly attaching polyethylene glycol to proteins and the use of this material in modifying the plasminogen activators urokinase- and tissue-type plasminogen activator are described. The characterisation and the reversible nature of these protein-polymer conjugates are discussed, and some of the in vitro properties of these modified enzymes are explored