Endogenic mediated synthesis of gold nanoparticles bearing bactericidal activity

AbstractThe present investigation aimed to synthesize gold nanoparticles using Pseudomonas fluorescens 417 inhabiting Coffea arabica L. Biologically synthesized gold nanoparticles were polydispersed in nature and characterized using hyphenated techniques such as UV-visible spectrophotometry, which ascertained characteristic peaks between 450nm and 650nm. Fourier transform infrared analysis predicted the functional groups present in the cell-free supernatant that mediated the synthesis and stabilization of gold nanoparticles. The crystalline nature of the gold nanoparticles was analyzed with X-ray diffraction techniques that displayed the Bragg's diffraction intensity. Transmission electron microscopy revealed the size of nanoparticles ranging from 5nm to 50nm, with most of them bearing a spherical shape. The study also revealed the bactericidal activity of synthesized nanoparticles against a panel of clinically significant pathogens. Maximum activity was observed against Pseudomonas aeroginosa followed by Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Klebsiella pneumoniae. The results obtained in the present investigation are promising for ecofriendly approaches for synthesis of gold nanoparticles bearing bactericidal activity that can act as an alternative to combat drug-resistant pathogens