Highly enantioselective bioreduction of ethyl 3-oxohexanoate
- Ramos, Aline de Souza
- Ribeiro, Joyce Benzaquem
- Lopes, Raquel de Oliveira
- Leite, Selma Gomes Ferreira
- Souza, Rodrigo Octavio Mendonça Alves de
Publication date
November 2011
DOI Publisher
Elsevier Ltd. ISSN
0040-4039 Citation count (estimate)
8Abstract
AbstractSeven wild-type microorganism strains were used to reduce ethyl 3-oxohexanoate to ethyl (R)-3-hydroxyhexanoate. Free cells of Kluyveromyces marxianus and Aspergillus niger led to higher than 99% of conversion with higher than 99% ee. After immobilization in calcium alginate spheres, cells of K. marxianus exhibited high enantioselectivity (>99% ee) and conversion level (99%) within 24h even if substrate was added at concentration of 10g/L (62mM)
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