Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system

AbstractTwelve bacterial clones containing complementary sequences to yeast tRNAArg3 were isolated from a gene library. The size of the yeast BamHI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT-3 was mapped, and the presence of a tRNAArg3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRANArg3A genes, this one is not linked to a tRNAAsp gene. In vitro transcription using a yeast extract produces a transcript of 76 ± 1 bases