AbstractLytechinus pictus sea urchin sperm express receptors for speract, a sperm-activating peptide derived from the homologous egg jelly coat. We found that the fluorescence of fluorophore-labeled, active, speract analogs is quenched upon receptor binding. This property allowed us to perform real-time measurements of speract–receptor interactions using intact sperm and to determine, for the first time, their association (kon) and dissociation (koff) rate constants. The high kon (2.4 × 107 M−1 s−1) and low koff (4.4 × 10−6 s−1 (95%) and 3.7 × 10−4 s−1 (5%)) can account for the sperm response to picomolar concentrations of speract. We also examined the influence of extracellular ions on speract–receptor interactions using the fluorescence q...
AbstractIon channels are deeply involved in sperm physiology. In sea urchin sperm cyclic nucleotide ...
Kisspeptin, the product of the KISS1 gene, plays an essential role in the regulation of spermatogene...
Sperm from the Atlantic purple sea urchin Arbacia punctulata has become an attractive model for stud...
AbstractSignal transduction initiated by the egg peptide, speract, in sea urchin sperm is not fully ...
AbstractSperm chemotaxis has an important role in fertilization. Most of our knowledge regarding thi...
AbstractSperact, a peptide from the egg jelly coat of certain sea urchin species, modulates sperm mo...
AbstractSperact, a decapeptide from Strongylocentrotus purpuratus sea urchin eggs, transiently stimu...
AbstractSpermatozoa of the starfish, Asterias amurensis, have a specific receptor for asterosap, a s...
AbstractSperact, an egg-derived sperm-activating peptide, induces changes in intracellular Ca2+, Na+...
AbstractPrevious studies have established that a recombinant protein fragment (45A) of the egg recep...
AbstractComponents of the sea urchin outer egg jelly layer such as speract drastically change second...
AbstractPeptides released from eggs of marine invertebrates play a central role in fertilization. Ab...
© 2004 Solzin et al. This article is distributed under the terms of the Creative Commons Attribution...
Sea urchin sperm motility can be activated by alkalinization of the internal pH, and previous studie...
The sperm protein bindin is responsible for the species-specific adhesion of the sperm to the egg. T...
AbstractIon channels are deeply involved in sperm physiology. In sea urchin sperm cyclic nucleotide ...
Kisspeptin, the product of the KISS1 gene, plays an essential role in the regulation of spermatogene...
Sperm from the Atlantic purple sea urchin Arbacia punctulata has become an attractive model for stud...
AbstractSignal transduction initiated by the egg peptide, speract, in sea urchin sperm is not fully ...
AbstractSperm chemotaxis has an important role in fertilization. Most of our knowledge regarding thi...
AbstractSperact, a peptide from the egg jelly coat of certain sea urchin species, modulates sperm mo...
AbstractSperact, a decapeptide from Strongylocentrotus purpuratus sea urchin eggs, transiently stimu...
AbstractSpermatozoa of the starfish, Asterias amurensis, have a specific receptor for asterosap, a s...
AbstractSperact, an egg-derived sperm-activating peptide, induces changes in intracellular Ca2+, Na+...
AbstractPrevious studies have established that a recombinant protein fragment (45A) of the egg recep...
AbstractComponents of the sea urchin outer egg jelly layer such as speract drastically change second...
AbstractPeptides released from eggs of marine invertebrates play a central role in fertilization. Ab...
© 2004 Solzin et al. This article is distributed under the terms of the Creative Commons Attribution...
Sea urchin sperm motility can be activated by alkalinization of the internal pH, and previous studie...
The sperm protein bindin is responsible for the species-specific adhesion of the sperm to the egg. T...
AbstractIon channels are deeply involved in sperm physiology. In sea urchin sperm cyclic nucleotide ...
Kisspeptin, the product of the KISS1 gene, plays an essential role in the regulation of spermatogene...
Sperm from the Atlantic purple sea urchin Arbacia punctulata has become an attractive model for stud...