The shrimp hyperglycemic hormone-like neuropeptide is encoded by multiple copies of genes arranged in a cluster1The DNA sequences of the MeCHH-like cDNA and four (43-1, 43-2, 43-3 and 43-4) out of six CHH-like genes have been submitted to the GenBank sequence database with the accession numbers AF109775, AF109776, AF109777, AF109778 and AF109779 respectively.1

AbstractThe crustacean hyperglycemic hormone (CHH) plays an important role in the regulation of glucose metabolism. We have cloned and sequenced several cDNAs encoding the preproCHH-like of the shrimp, Metapenaeus ensis. The preproCHH-like peptide of the shrimp consists of a signal peptide, a CHH precursor-like peptide (CPRP) and the CHH-like peptide. Comparative analysis revealed that the signal peptide and the CPRP of the shrimp peptide are the shortest among all the CHHs reported. MeCHH-like is expressed in the eyestalk, but it is not expressed in the heart, hepatopancreas, muscle, nerve cord and pre-hatch embryo. To study the structural organization of the shrimp CHH-like gene, we have screened the genomic DNA library constructed from one shrimp. Three groups of overlapping genomic clones have been isolated. The results from both genomic Southern blot analysis and library screening indicate that the shrimp genome contains at least six copies of the CHH-like genes arranged in a cluster on the chromosome. The size of the CHH-like genes is 1.5–2.1 kb. DNA sequence determinations indicate that the CHH-like genes share 98–100% amino acid sequence identity. There are three exons and two introns in each CHH-like gene. The first intron separates the signal peptide and the second intron separates the mature peptide in the coding region. The 150–200 bp of the upstream 5′ flanking region of the CHH-like genes contains promoters with characteristics similar to most eukaryotic genes. Several putative cis-acting elements are also identified in the first 400 bp 5′ end upstream region. The organization of the shrimp CHH-like genes is similar to that of the molt inhibiting hormone gene of the same shrimp and the crab, Charybdis feriatus