Analytical characterization and clinical evaluation of an enzyme-linked immunosorbent assay for measurement of afamin in human plasma

Dieplinger, Benjamin
Egger, Margot
Gabriel, Christian
Poelz, Werner
Morandell, Elisabeth
Seeber, Beata
Kronenberg, Florian
Haltmayer, Meinhard
Mueller, Thomas
Dieplinger, Hans

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Publication date

October 2013

DOI

10.1016/j.cca.2013.08.016

Publisher

Published by Elsevier B.V.

ISSN

0009-8981

Citation count (estimate)

Abstract

AbstractBackgroundComparative proteomics has recently identified afamin, the newest member of the albumin gene family, as a potential biomarker for ovarian cancer. The aim of this study was the analytical and clinical evaluation of a sandwich enzyme-linked immunosorbent assay for the determination of afamin in human plasma.MethodsWe evaluated precision, linearity, and detection limit of the assay, analyte stability and biological variability, determined reference values and quantified afamin concentrations in various diseases.ResultsWithin-run and total coefficients of variation were <10%. The method was linear across the tested measurement range. Detection limit was 7mg/L for the assay. The analyte was stable for 24h at room temperature, f...