Combination of 3D Extruded-based Poly (ɛ-caprolactone) Scaffolds with Mesenchymal Stem/Stromal Cells: Strategy Optimization

AbstractThis study uses a combination of 3D extruded-based biodegradable scaffolds and human bone marrow-derived mesenchymal stem/stromal cells to understand how scaffold properties can contribute for efficient cell seeding, proliferation and migration. Highly interconnected porous poly(ɛ-caprolactone) scaffolds were produced by a layer-by-layer extrusion. Two configurations were studied with larger (390μm) or smaller pore size (190μm) at the centre. MSC, seeded on the top centre of the scaffold surface, increased 5 folds after 13 days of cultivation for both configurations, allowing us to conclude that in this range of pore sizes pose no limitations for cell migration are observed