AbstractHydrogen peroxide (H2O2) is known to both induce and inhibit apoptosis, however the mechanisms are unclear. We found that H2O2 inhibited the activity of recombinant caspase-3 and caspase-8, half-inhibition occurring at about 17 μM H2O2. This inhibition was both prevented and reversed by dithiothreitol while glutathione had little protective effect. 100–200 μM H2O2 added to macrophages after induction of caspase activation by nitric oxide or serum withdrawal substantially inhibited caspase activity. Activation of H2O2-producing NADPH oxidase in macrophages also caused catalase-sensitive inactivation of cellular caspases. The data suggest that the activity of caspases in cells can be directly but reversibly inhibited by H2O2
A 3-hr exposure of U937 cells to hydrogen peroxide (H2O2) followed by a 6-hr posttreatment incubatio...
AbstractPeroxides generated on peptides and proteins within cells, as a result of radical attack or ...
We have studied the relationships existing between delayed formation of H2O2 and activation of cytos...
AbstractHydrogen peroxide (H2O2) is known to both induce and inhibit apoptosis, however the mechanis...
AbstractThe induction of apoptosis in Jurkat T-lymphocytes with 50 μM hydrogen peroxide was associat...
AbstractCaspases have an active site cysteine whose oxidation blocks catalytic activity. Caspase act...
AbstractThe induction of apoptosis in Jurkat T-lymphocytes with 50 μM hydrogen peroxide was associat...
AbstractHydrogen peroxide (H2O2) is considered to be a mediator of apoptotic cell death but the mech...
Caspases have an active site cysteine whose oxidation blocks catalytic activity. Caspase activity, m...
Treatment with 0.2mM hydrogen peroxide (H2O2) or with 0.5mM cisplatin caused caspase-9 and caspase-3...
Caspases have an active site cysteine whose oxidation blocks catalytic activity. Caspase activity, m...
A 3-hr exposure of U937 cells to hydrogen peroxide (H2O2) followed by a 6-hr posttreatment incubatio...
Treatment with 0.2mM hydrogen peroxide (H2O2) or with 0.5mM cisplatin caused caspase-9 and caspase-3...
Treatment with 0.2mM hydrogen peroxide (H2O2) or with 0.5mM cisplatin caused caspase-9 and caspase-3...
A 3-hr exposure of U937 cells to hydrogen peroxide (H2O2) followed by a 6-hr posttreatment incubatio...
A 3-hr exposure of U937 cells to hydrogen peroxide (H2O2) followed by a 6-hr posttreatment incubatio...
AbstractPeroxides generated on peptides and proteins within cells, as a result of radical attack or ...
We have studied the relationships existing between delayed formation of H2O2 and activation of cytos...
AbstractHydrogen peroxide (H2O2) is known to both induce and inhibit apoptosis, however the mechanis...
AbstractThe induction of apoptosis in Jurkat T-lymphocytes with 50 μM hydrogen peroxide was associat...
AbstractCaspases have an active site cysteine whose oxidation blocks catalytic activity. Caspase act...
AbstractThe induction of apoptosis in Jurkat T-lymphocytes with 50 μM hydrogen peroxide was associat...
AbstractHydrogen peroxide (H2O2) is considered to be a mediator of apoptotic cell death but the mech...
Caspases have an active site cysteine whose oxidation blocks catalytic activity. Caspase activity, m...
Treatment with 0.2mM hydrogen peroxide (H2O2) or with 0.5mM cisplatin caused caspase-9 and caspase-3...
Caspases have an active site cysteine whose oxidation blocks catalytic activity. Caspase activity, m...
A 3-hr exposure of U937 cells to hydrogen peroxide (H2O2) followed by a 6-hr posttreatment incubatio...
Treatment with 0.2mM hydrogen peroxide (H2O2) or with 0.5mM cisplatin caused caspase-9 and caspase-3...
Treatment with 0.2mM hydrogen peroxide (H2O2) or with 0.5mM cisplatin caused caspase-9 and caspase-3...
A 3-hr exposure of U937 cells to hydrogen peroxide (H2O2) followed by a 6-hr posttreatment incubatio...
A 3-hr exposure of U937 cells to hydrogen peroxide (H2O2) followed by a 6-hr posttreatment incubatio...
AbstractPeroxides generated on peptides and proteins within cells, as a result of radical attack or ...
We have studied the relationships existing between delayed formation of H2O2 and activation of cytos...