The homologous sequences observed for many calcium binding proteins such as parvalbumin, troponin C, the myosin light chains, and calmodulin has lead to the hypothesis that these proteins have homologous structures at the level of their calcium binding sites. This paper discusses the development of a nuclear magnetic resonance (NMR) technique which will enable us to test this structural hypothesis in solution. The technique involves the substitution of a paramagnetic lanthanide ion for the calcium ion which results in lanthanide induced shifts and broadening in the 1H NMR spectrum of the protein. These shifts are sensitive monitors of the precise geometrical orientation of each proton nucleus relative to the metal. The values of several par...
Nuclear magnetic resonance (NMR) spectroscopy was used in order to investigate the relationships bet...
The kinetics of lanthanide (III) exchange for calcium(II) in the C-terminal EF-hand of the protein c...
Understanding biological phenomena at atomic resolution is one of the keys to modern drug design. In...
Lanthanide-shifted 1H nuclear magnetic resonance (NMR) spectroscopy has been used to compare the str...
Paramagnetic NMR has drawn interest as a technique for extending the range of systems that can be in...
AbstractThis work shows that the partial replacement of diamagnetic Ca2+ by paramagnetic Tb3+ in Ca2...
A strategy for the accurate determination of protein solution structures starting from X-ray data an...
The 1H NMR spectra of carp parvalbumin saturated with Ca2+, Cd2+, La3+ and Lu3+ were compared, using...
Rational drug design depends on the knowledge of the three-dimensional (3D) structure of complexes b...
The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the...
SummarySite-specific attachment of paramagnetic lanthanide ions to a protein generates pseudocontact...
The advent of different lanthanide-binding reagents has made site-specific labelling of proteins wit...
Abstract High resolution 1H nuclear magnetic resonance spectroscopy and optical stopped-flow techniq...
The application of paramagnetic lanthanoid chelating tags for the localization of ligands within bio...
In this dissertation we show how to push the boundaries of structure elucidation using tensorial NMR...
Nuclear magnetic resonance (NMR) spectroscopy was used in order to investigate the relationships bet...
The kinetics of lanthanide (III) exchange for calcium(II) in the C-terminal EF-hand of the protein c...
Understanding biological phenomena at atomic resolution is one of the keys to modern drug design. In...
Lanthanide-shifted 1H nuclear magnetic resonance (NMR) spectroscopy has been used to compare the str...
Paramagnetic NMR has drawn interest as a technique for extending the range of systems that can be in...
AbstractThis work shows that the partial replacement of diamagnetic Ca2+ by paramagnetic Tb3+ in Ca2...
A strategy for the accurate determination of protein solution structures starting from X-ray data an...
The 1H NMR spectra of carp parvalbumin saturated with Ca2+, Cd2+, La3+ and Lu3+ were compared, using...
Rational drug design depends on the knowledge of the three-dimensional (3D) structure of complexes b...
The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the...
SummarySite-specific attachment of paramagnetic lanthanide ions to a protein generates pseudocontact...
The advent of different lanthanide-binding reagents has made site-specific labelling of proteins wit...
Abstract High resolution 1H nuclear magnetic resonance spectroscopy and optical stopped-flow techniq...
The application of paramagnetic lanthanoid chelating tags for the localization of ligands within bio...
In this dissertation we show how to push the boundaries of structure elucidation using tensorial NMR...
Nuclear magnetic resonance (NMR) spectroscopy was used in order to investigate the relationships bet...
The kinetics of lanthanide (III) exchange for calcium(II) in the C-terminal EF-hand of the protein c...
Understanding biological phenomena at atomic resolution is one of the keys to modern drug design. In...