The off-line coupling of an isoelectric trapping device termed membrane separated wells for isoelectric focusing and trapping (MSWIFT) to mass spectrometry-based proteomic studies is described. The MSWIFT is a high capacity, high-throughput, mass spectrometry-compatible isoelectric trapping device that provides isoelectric point (pI)-based separations of complex mixtures of peptides. In MSWIFT, separation and analyte trapping are achieved by migrating the peptide ions through membranes having fixed pH values until the peptide pI is bracketed by the pH values of adjacent membranes. The pH values of the membranes can be tuned, thus affording a high degree of experimental flexibility. Specific advantages of using MSWIFT for sample prefractiona...
Motivation: In any macromolecular polyprotic system - for example protein, DNA or RNA - the isoelect...
Abstract Introduction The vast difference in the abundance of different proteins in biological sampl...
We introduce an online multiple-junction capillary isoelectric focusing fractionator (OMJ-CIEF) for ...
The off-line coupling of an isoelectric trapping device termed membrane separated wells for isoelect...
Mass spectrometry (MS) has played a major role in the proteomic analysis of an array of biological ...
In this paper, we are evaluating the strategy of sorting peptides/proteins based on the charge to ma...
In shotgun proteomics, protein mixtures are proteolytically digested before tandem mass spectrometry...
Recently, we introduced an online multijunction capillary isoelectric focusing (OMJ-CIEF) fractionat...
Proteomics is the systematic analysis of proteins and their expression patterns in biological syst...
To increase sensitivity and analytical depth in shotgun proteomics, prefractionation of complex samp...
Proteomics is an increasingly important area of biological research and has gathered much attention...
Imaging Mass Spectrometry (IMS) has emerged as a powerful technique in the field of proteomics. The ...
Introduction. The vast difference in the abundance of different proteins in biological samples limit...
AbstractA reliable capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry s...
A stepwise mobilization strategy has been developed for the elution of complex protein mixtures, sep...
Motivation: In any macromolecular polyprotic system - for example protein, DNA or RNA - the isoelect...
Abstract Introduction The vast difference in the abundance of different proteins in biological sampl...
We introduce an online multiple-junction capillary isoelectric focusing fractionator (OMJ-CIEF) for ...
The off-line coupling of an isoelectric trapping device termed membrane separated wells for isoelect...
Mass spectrometry (MS) has played a major role in the proteomic analysis of an array of biological ...
In this paper, we are evaluating the strategy of sorting peptides/proteins based on the charge to ma...
In shotgun proteomics, protein mixtures are proteolytically digested before tandem mass spectrometry...
Recently, we introduced an online multijunction capillary isoelectric focusing (OMJ-CIEF) fractionat...
Proteomics is the systematic analysis of proteins and their expression patterns in biological syst...
To increase sensitivity and analytical depth in shotgun proteomics, prefractionation of complex samp...
Proteomics is an increasingly important area of biological research and has gathered much attention...
Imaging Mass Spectrometry (IMS) has emerged as a powerful technique in the field of proteomics. The ...
Introduction. The vast difference in the abundance of different proteins in biological samples limit...
AbstractA reliable capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry s...
A stepwise mobilization strategy has been developed for the elution of complex protein mixtures, sep...
Motivation: In any macromolecular polyprotic system - for example protein, DNA or RNA - the isoelect...
Abstract Introduction The vast difference in the abundance of different proteins in biological sampl...
We introduce an online multiple-junction capillary isoelectric focusing fractionator (OMJ-CIEF) for ...