Evaluation of biological control agents for mosquitoes control in artificial breeding places

ObjectiveTo evaluate the entomological impact of chlorpyrifos-methyl, Bacillus thuringiensis, and Gambusia affinis on mosquitoes control in artificial breeding places.MethodsA Latin square design with 4 replicates was performed in order to evaluate the efficacy of chlorpyrifosmethyl, Bacillus thuringiensis, and Gambusia affinis on larva. The larvicide was applied at the dosage of 100 mg a.h/ha, Bacillus thuringiensis at the recommended dosage and 10 fishes per m2 were applied at 1×1 m2 artificial breeding sites. The larval densities for both anopheline and culicine were counted according to larvae /10 dippers prior and 24 h after application.ResultsAll three control agents are effective for mosquito density reduction, and the difference between the three agents and the control is significant (P<0.05). There is also significant difference among chlorpyrifos-methyl, Bacillus thuringiensis and Gambusia affinis. Bacillus thuringiensis exhibited more reduction on mosquito larval density than fish and larvicide (P