Tensile Strength of the Chromaffin Granule Membrane

Catecholamine release from chromaffin granules, suspended in sucrose solutions of various osmotic strengths, was determined at different temperatures between 2° and 44°C. Dynamic measurements showed that steady state is achieved within 15min of incubation at all temperatures. The effect of temperature on the release was established in terms of the median granular fragility (MGF) defined as the concentration of sucrose solution causing 50% lysis. The MGF was determined as the inflection point of the Gaussian distribution of granular fragility. The MGF was found to decrease with fall in temperature implying a corresponding increase of the tensile strength of the vesicle membrane. Critical resultant forces at lysis were calculated and found to vary from 8.2 dyn/cm at 2°C to 4.2 dyn/cm at 44°C. These compare well with tensions at lysis found earlier for erythrocytes