SummaryBackgroundGlobally, disinfectants are widely used to intervene in the dissemination of Cryptosporidium oocysts. However, extensive investigations of oocyst inactivation by various disinfectants are not feasible due to the limitations imposed by animal infectivity methods. Molecular techniques provide an alternative strategy; however, non-metabolic genes have been used as markers for determining viability/infectivity.MethodsIn this study we used amyloglucosidase (AG) – a metabolic protein – as a marker to determine viability/infectivity of Cryptosporidium. Oocysts were exposed to 6% hydrogen peroxide for 2min. Samples were analyzed by cell culture polymerase chain reaction (CC-PCR) using PCR primers specific for heat shock protein 70 ...
Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens...
Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens...
Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children i...
SummaryBackgroundGlobally, disinfectants are widely used to intervene in the dissemination of Crypto...
The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long pe...
Outbreaks of cryptosporidiosis have been traced to Cryptosporidium oocysts in finished drinking wate...
Viability estimation of the highly resistant oocysts of Cryptosporidium remains a key issue for the ...
Cryptosporidium parvum is an obligatory intracellular parasitic protist that belongs to the phylum A...
Cryptosporidiosis is a major cause of diarrheal illness worldwide and characterized by several daily...
Several in vitro surrogates have been developed as convenient, user-friendly alternatives to mouse i...
Cryptosporidium is one of the important causative agents of gastrointestinal illness in humans. Cryp...
The occurrence of Cryptosporidium oocysts in drinking source water can present a serious public heal...
Quantitative real-time polymerase chain reaction (qPCR) assays to detect Cryptosporidium oocysts in ...
The protozoan Cryptosporidium is notorious for its resistance to chlorine disinfection, a mainstay o...
The performance of polymerase chain reaction (PCR) procedures for the detection of Cryptosporidium p...
Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens...
Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens...
Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children i...
SummaryBackgroundGlobally, disinfectants are widely used to intervene in the dissemination of Crypto...
The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long pe...
Outbreaks of cryptosporidiosis have been traced to Cryptosporidium oocysts in finished drinking wate...
Viability estimation of the highly resistant oocysts of Cryptosporidium remains a key issue for the ...
Cryptosporidium parvum is an obligatory intracellular parasitic protist that belongs to the phylum A...
Cryptosporidiosis is a major cause of diarrheal illness worldwide and characterized by several daily...
Several in vitro surrogates have been developed as convenient, user-friendly alternatives to mouse i...
Cryptosporidium is one of the important causative agents of gastrointestinal illness in humans. Cryp...
The occurrence of Cryptosporidium oocysts in drinking source water can present a serious public heal...
Quantitative real-time polymerase chain reaction (qPCR) assays to detect Cryptosporidium oocysts in ...
The protozoan Cryptosporidium is notorious for its resistance to chlorine disinfection, a mainstay o...
The performance of polymerase chain reaction (PCR) procedures for the detection of Cryptosporidium p...
Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens...
Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens...
Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children i...