Recoverin mediates the calcium effect upon rhodopsin phosphorylation and cGMP hydrolysis in bovine retina rod cells
- Gorodovikova, Elena N.
- Gimelbrant, Alexandr A.
- Senin, Ivan I.
- Philippov, Pavel P.
Publication date
August 1994
DOI Publisher
Published by Elsevier B.V. ISSN
0014-5793Abstract
AbstractRhodopsin phosphorylation and in consequence cGMP hydrolysis in bovine rod outer segments are Ca2+ dependent in the presence of ATP. The level of rhodopsin phosphorylation decreases and the lifetime of active phosphodiesterase increases when the free [Ca2+] is raised from <1 nM to about 1 μM; in both cases the half-maximal effect was observed at 140–170 nM of free Ca2+. Antibodies to recoverin reverse both effects at high [Ca2+] but have no influence at low [Ca2+]. We conclude that the Ca2+ effects observed are mediated by recoverin which inhibits rhodopsin kinase at a high Ca2+ level
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