Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry
- Kamleh, M.A.
- Hobani, Y.
- Dow, J.A.T.
- Watson, D.G.
Publication date
August 2008
DOI Publisher
Federation of European Biochemical Societies. Published by Elsevier B.V.Abstract
AbstractHydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy (ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the technology by detecting the same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected changes were detected in each of the tryptophan, arginine, pyrimidine and glycerophospholipid metabolism pathways. The rosy mutation thus ramifies far more widely than previously detected
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