Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation
- Wang, Wenbo
- Kutny, Peter M.
- Byers, Shannon L.
- Longstaff, Charles J.
- DaCosta, Michael J.
- Pang, Changhong
- Zhang, Yingfan
- Taft, Robert A.
- Buaas, Frank W.
- Wang, Haoyi
Publication date
May 2016
DOI Publisher
, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. ISSN
1673-8527 Citation count (estimate)
24Abstract
AbstractPreviously we established Zygote Electroporation of Nucleases (ZEN) technology as an efficient and high-throughput way to generate genetically modified mouse models. However, there were significant variations of the targeting efficiency among different genomic loci using our previously published protocol. In this study, we improved the ZEN technology by delivering Cas9 protein into mouse zygotes through a series of electroporation. Using this approach, we were able to introduce precise nucleotide substitutions, large segment deletion and short segment insertion into targeted loci with high efficiency
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