Fluorescence Lifetime Imaging Provides Enhanced Contrast when Imaging the Phase-Sensitive Dye di-4-ANEPPDHQ in Model Membranes and Live Cells
- Owen, Dylan M.
- Lanigan, Peter M.P.
- Dunsby, Christopher
- Munro, Ian
- Grant, David
- Neil, Mark A.A.
- French, Paul M.W.
- Magee, Anthony I.
Publication date
June 2006
DOI Publisher
The Biophysical Society. Published by Elsevier Inc.Abstract
AbstractWe apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-β-cyclodextrin
Add to ORKG