AbstractC-Raf is a member of the Ras–Raf–MEK–ERK mitogen-activated protein kinase (MAPK) signaling pathway that plays key roles in diverse physiological processes and is upregulated in many human cancers. C-Raf activation involves binding to Ras, increased phosphorylation and interactions with co-factors. Here, we describe a Ras-independent in vivo pathway for C-Raf activation by its downstream target MEK. Using 32P-metabolic labeling and 2D-phosphopeptide mapping experiments, we show that MEK increases C-Raf phosphorylation by up-to 10-fold. This increase was associated with C-Raf kinase activation, matching the activity seen with growth factor stimulation. Consequently, coexpression of wildtype C-Raf and MEK was sufficient for full and co...
Integrin-mediated adhesion to the extracellular matrix activates the canonical mitogen-activated pro...
Activated Ras, but not Raf, causes transformation of RIE-1 epithelial cells, supporting the importan...
AbstractThe Ras-Raf-MAPK pathway regulates diverse physiological processes by transmitting signals f...
AbstractC-Raf is a member of the Ras–Raf–MEK–ERK mitogen-activated protein kinase (MAPK) signaling p...
AbstractOver 30 mutations of the B-RAF gene associated with human cancers have been identified, the ...
Substantial evidence supports a critical role for the activation of the Raf-1/MEK/mitogen-activated ...
Substantial evidence supports a critical role for the activation of the Raf-1/MEK/mitogen-activated ...
ation of a number of key substrates including other protein The c-Raf-1 protein kinase plays a criti...
RAF research is booming since the discovery of mutant B-RAF in ∼8% of human cancer. One reason for t...
AbstractThe Raf/Mek/Erk signaling pathway, activated downstream of Ras primarily to promote prolifer...
The Ras-Raf-MEK-ERK signaling pathway, the first well-established MAPK pathway, plays essential role...
The MAP Kinase pathway is a key signaling mechanism that regulates many cellular functions such as c...
AbstractThe Raf/MEK/ERK pathway is a conserved signaling module controlling cell growth, proliferati...
The potent transforming activity of membrane-targeted Raf-1 (Raf-CAAX) suggests that Ras transformat...
Growth factor stimulated receptor tyrosine kinases activate a protein kinase cascade via the serine/...
Integrin-mediated adhesion to the extracellular matrix activates the canonical mitogen-activated pro...
Activated Ras, but not Raf, causes transformation of RIE-1 epithelial cells, supporting the importan...
AbstractThe Ras-Raf-MAPK pathway regulates diverse physiological processes by transmitting signals f...
AbstractC-Raf is a member of the Ras–Raf–MEK–ERK mitogen-activated protein kinase (MAPK) signaling p...
AbstractOver 30 mutations of the B-RAF gene associated with human cancers have been identified, the ...
Substantial evidence supports a critical role for the activation of the Raf-1/MEK/mitogen-activated ...
Substantial evidence supports a critical role for the activation of the Raf-1/MEK/mitogen-activated ...
ation of a number of key substrates including other protein The c-Raf-1 protein kinase plays a criti...
RAF research is booming since the discovery of mutant B-RAF in ∼8% of human cancer. One reason for t...
AbstractThe Raf/Mek/Erk signaling pathway, activated downstream of Ras primarily to promote prolifer...
The Ras-Raf-MEK-ERK signaling pathway, the first well-established MAPK pathway, plays essential role...
The MAP Kinase pathway is a key signaling mechanism that regulates many cellular functions such as c...
AbstractThe Raf/MEK/ERK pathway is a conserved signaling module controlling cell growth, proliferati...
The potent transforming activity of membrane-targeted Raf-1 (Raf-CAAX) suggests that Ras transformat...
Growth factor stimulated receptor tyrosine kinases activate a protein kinase cascade via the serine/...
Integrin-mediated adhesion to the extracellular matrix activates the canonical mitogen-activated pro...
Activated Ras, but not Raf, causes transformation of RIE-1 epithelial cells, supporting the importan...
AbstractThe Ras-Raf-MAPK pathway regulates diverse physiological processes by transmitting signals f...