AbstractSelectins mediate circulatory leukocyte trafficking to sites of inflammation and trauma, and the extracellular microenvironments at these sites often become acidic. In this study, we investigated the influence of slightly acidic pH on the binding dynamics of selectins (P-, L-, and E-selectin) to P-selectin glycoprotein ligand-1 (PSGL-1) via computational modeling (molecular dynamics) and experimental rolling assays under shear in vitro. The P-selectin/PSGL-1 binding is strengthened at acidic pH, as evidenced by the formation of a new hydrogen bond (seen computationally) and the observed decrease in the rolling velocities of model cells. In the case of L-selectin/PSGL-1 binding dynamics, the binding strength and frequency increase at...
SummaryThe selectins and their ligands are required for leukocyte extravasation during inflammation....
Selectins play a critical role in initiating leukocyte binding to vascular endothelium. In addition,...
AbstractL-selectin-mediated leukocyte rolling has been proposed to require a high rate of bond forma...
AbstractSelectins mediate circulatory leukocyte trafficking to sites of inflammation and trauma, and...
AbstractP-, E- and L-selectin constitute a family of cell adhesion receptors that mediate the initia...
© 2008 by the Biophysical SocietyDOI: 10.1529/biophysj.107.114447Binding of selectins to P-selectin ...
AbstractA laser trap was used to compare the load-dependent binding kinetics between truncated P- an...
AbstractP-selectin glycoprotein ligand-1 (PSGL-1) is a large (240 kDa) glycoprotein found on the sur...
AbstractBinding of selectins to P-selectin glycoprotein ligand-1 (PSGL-1) mediates tethering and rol...
Inflammation is a key physiological process, important in infections, autoimmune diseases, and chron...
AbstractDuring inflammation, flowing leukocytes tether to and roll on vascular surfaces through the ...
In response to vascular damage, P-selectin molecules are secreted onto the surface of cells that lin...
Adhesion of leukocytes to endothelium is a critical step in the inflammatory response. P-selectin, a...
AbstractA cell-scaled microbead system was used to analyze the force-dependent kinetics of P-selecti...
© 2013 Zhang et al. This is an open-access article distributed under the terms of the Creative Commo...
SummaryThe selectins and their ligands are required for leukocyte extravasation during inflammation....
Selectins play a critical role in initiating leukocyte binding to vascular endothelium. In addition,...
AbstractL-selectin-mediated leukocyte rolling has been proposed to require a high rate of bond forma...
AbstractSelectins mediate circulatory leukocyte trafficking to sites of inflammation and trauma, and...
AbstractP-, E- and L-selectin constitute a family of cell adhesion receptors that mediate the initia...
© 2008 by the Biophysical SocietyDOI: 10.1529/biophysj.107.114447Binding of selectins to P-selectin ...
AbstractA laser trap was used to compare the load-dependent binding kinetics between truncated P- an...
AbstractP-selectin glycoprotein ligand-1 (PSGL-1) is a large (240 kDa) glycoprotein found on the sur...
AbstractBinding of selectins to P-selectin glycoprotein ligand-1 (PSGL-1) mediates tethering and rol...
Inflammation is a key physiological process, important in infections, autoimmune diseases, and chron...
AbstractDuring inflammation, flowing leukocytes tether to and roll on vascular surfaces through the ...
In response to vascular damage, P-selectin molecules are secreted onto the surface of cells that lin...
Adhesion of leukocytes to endothelium is a critical step in the inflammatory response. P-selectin, a...
AbstractA cell-scaled microbead system was used to analyze the force-dependent kinetics of P-selecti...
© 2013 Zhang et al. This is an open-access article distributed under the terms of the Creative Commo...
SummaryThe selectins and their ligands are required for leukocyte extravasation during inflammation....
Selectins play a critical role in initiating leukocyte binding to vascular endothelium. In addition,...
AbstractL-selectin-mediated leukocyte rolling has been proposed to require a high rate of bond forma...