Effects of polar carotenoids on the shape of the hydrophobic barrier of phospholipid bilayers

AbstractThe value of Az (z-component of the hyperfine interaction tensor) obtained directly from X-band EPR spectra of stearic acid spin labels and tempocholine dipalmitoylphosphatidic acid ester in frozen suspension of phosphatidylcholine (PC) membranes has been used as a hydrophobicity parameter. Using probes with the nitroxide moiety at various depths in the membrane, the shape of the hydrophobic barrier, which is determined by the extent of water penetration into the membrane, has been estimated. Incorporation of 10mol% polar carotenoids, zeaxanthin, violaxanthin, or lutein into the saturated PC bilayer significantly increases the hydrophobicity of the membrane interior but decreases hydrophobicity (increases water penetration) in the polar headgroup region. Hydrophobicity at the membrane center increases from the level of propanol–pentanol, which have dielectric constants of 10–20, to the level of dipropylamine, with a dielectric constant close to 3. Longer alkyl chains decrease the effect of polar carotenoids in the polar headgroup region, but not in the central hydrophobic region. In an unsaturated egg yolk PC membrane, polar carotenoids were found to increase the hydrophobicity of the membrane interior to a higher level than in saturated PC membranes. At the membrane center hydrophobicity reaches the level close to pure hexane (ϵ≃2). The above results were confirmed by studying accessibility of Fe(CN)3−6 ion dissolved in water into dimyristoyl-PC-lutein membranes at 30°C. Obtained hydrophobicity profiles correlate well with permeability data for water in the literature