Recognition of the let-7g miRNA precursor by human Lin28B
- Shaik Syed Ali, P.
- Ghoshdastider, Umesh
- Hoffmann, Jan
- Brutschy, Bernd
- Filipek, Slawomir
Publication date
November 2012
DOI Publisher
Federation of European Biochemical Societies. Published by Elsevier B.V. ISSN
0014-5793 Citation count (estimate)
14Abstract
AbstractMammalian homologs of lin28: Lin28 and Lin28B block the post-transcriptional processing of the let-7 family of miRNAs. We report that in vitro the terminal stem-loop region of the let-7g miRNA precursor (pre-let-7g) required to bind Lin28B is restricted to 24 nucleotides (nt) including the 3′ GGAG motif. Additionally, full length Lin28B is required for efficient binding to pre-let-7g and the stoichiometry of the complex is 1:1. Molecular dynamics (MD) simulations reveal the interactions of the pre-let-7g stem-loop and the GGAG motif in the stem region to the cold shock domain (CSD) and to the zinc knuckle domain (ZKD) of Lin28B, respectively
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