AbstractCD studies have shown that five tryptophan to phenylalanine (W→F) mutants of eukaryotic initiation factor-4E (eIF-4E) contain low amounts of α-helix, the main elements of secondary structure being β-sheets/turns and aperiodic regions. Interactions with the cap analog m7GpppG are accompanied by changes in overall secondary structure which include reductions, and in one case an increase in α-helix content, as well as increases in total β-structure (3 mutant forms) and decreases in total β-structure (2 mutant forms). These changes may also involve more significant perturbations of localized regions containing phenylalanine residues either involved in nucleotide binding, or close to the nucleotide-binding site. Measurements of intrinsic...
Eukaryotic initiation factor 5A (eIF5A) is the only protein in nature that contains hypusine, an unu...
The eukaryotic translation initiation factor 4A (eIF-4A) possesses an in vitro helicase activity tha...
The cap-binding protein eIF4E is the first in a chain of translation initiation factors that recruit...
Eukaryotic initiation factor 2A (eIF2A) has been shown to direct binding of the initiator methionyl-...
AbstractActin contains four tryptophan residues, W79, W86, W340, and W356, all located in subdomain ...
Translation initiation accuracy in the cell is of paramount importance, as beginning polypeptide syn...
Eukaryotic initiation factor (eIF) 4F binding to mRNA is the first committed step in cap-dependent p...
Translation initiation factors eIF4A and eIF4G form, together with the cap-binding factor eIF4E, the...
Eukaryotic translation initiation factor 5A (eIF5A) is a protein that is highly conserved and essent...
Protein synthesis is principally regulated at the initiation stage in which eIF4F complex plays an i...
AbstractEukaryotic initiation factor 4E (eIF4E) binds the 5′-cap of eukaryotic mRNAs and overexpress...
AbstractThe X-ray structure of the eukaryotic translation initiation factor 4E (eIF4E), bound to 7-m...
SummaryRecognition of the mRNA 5′ m7G(5′)ppp(5′)N cap is key to translation initiation for most euka...
Protein synthesis can be divided into three distinct subprocesses, namely initiation, elongation and...
In eukaryotes, the m(7)GpppN cap structure is added to all nascent RNA polymerase II transcripts, an...
Eukaryotic initiation factor 5A (eIF5A) is the only protein in nature that contains hypusine, an unu...
The eukaryotic translation initiation factor 4A (eIF-4A) possesses an in vitro helicase activity tha...
The cap-binding protein eIF4E is the first in a chain of translation initiation factors that recruit...
Eukaryotic initiation factor 2A (eIF2A) has been shown to direct binding of the initiator methionyl-...
AbstractActin contains four tryptophan residues, W79, W86, W340, and W356, all located in subdomain ...
Translation initiation accuracy in the cell is of paramount importance, as beginning polypeptide syn...
Eukaryotic initiation factor (eIF) 4F binding to mRNA is the first committed step in cap-dependent p...
Translation initiation factors eIF4A and eIF4G form, together with the cap-binding factor eIF4E, the...
Eukaryotic translation initiation factor 5A (eIF5A) is a protein that is highly conserved and essent...
Protein synthesis is principally regulated at the initiation stage in which eIF4F complex plays an i...
AbstractEukaryotic initiation factor 4E (eIF4E) binds the 5′-cap of eukaryotic mRNAs and overexpress...
AbstractThe X-ray structure of the eukaryotic translation initiation factor 4E (eIF4E), bound to 7-m...
SummaryRecognition of the mRNA 5′ m7G(5′)ppp(5′)N cap is key to translation initiation for most euka...
Protein synthesis can be divided into three distinct subprocesses, namely initiation, elongation and...
In eukaryotes, the m(7)GpppN cap structure is added to all nascent RNA polymerase II transcripts, an...
Eukaryotic initiation factor 5A (eIF5A) is the only protein in nature that contains hypusine, an unu...
The eukaryotic translation initiation factor 4A (eIF-4A) possesses an in vitro helicase activity tha...
The cap-binding protein eIF4E is the first in a chain of translation initiation factors that recruit...