Fraktionierung handelsüblichen insulins. Gewinnung und eigenschaften einer hochmolekularen, insulin-ähnlichen komponente

AbstractA component with high molecular weight is prepared from commercial bovine insulin by gel filtration on Sephadex G 50. The substance has little biological and immunological activity in comparison to insulin. The activity is not changed by incubation with trypsin. It associates in 1 M CH3COOH and possesses a molecular weight of 28,500 as shown by ultracentrifugation. The ability of the component to precipitate with bovine insulin antibodies demonstrates that it contains immunologically active insulin-like proteins and is not a homogenous substance