Protein synthesis by isolated chloroplasts of Chlamydomonas reinhardii

AbstractChloroplasts which have been isolated from the cell wall mutant cw15-2 of Chlamydomonas reinhardii are active in light-dependent, in organello protein synthesis. Certain characteristics of protein synthesis in this system were tested, such as time course of amino acid incorporation, sensitivities to Mg2+ as well as to specific inhibitors, and found to be typical of chloroplasts isolated from higher plants. The major products made were identified as the large subunit of ribulose-1,5-biphosphate carboxylase and the 32-kDa rapidly labelled membrane protein