SummaryActivation of the DNA replication checkpoint by the ATR kinase requires protein interactions mediated by the ATR-activating protein, TopBP1. Accumulation of TopBP1 at stalled replication forks requires the interaction of TopBP1 BRCT5 with the phosphorylated SDT repeats of the adaptor protein MDC1. Here, we present the X-ray crystal structures of the tandem BRCT4/5 domains of TopBP1 free and in complex with a MDC1 consensus pSDpT phosphopeptide. TopBP1 BRCT4/5 adopts a variant BRCT-BRCT packing interface and recognizes its target peptide in a manner distinct from that observed in previous tandem BRCT- peptide structures. The phosphate-binding pocket and positively charged residues in a variant loop in BRCT5 present an extended binding...
In mitosis, cells inactivate DNA double-strand break (DSB) repair pathways to preserve genome stabil...
TopBP1, acting in concert with DNA containing bulky base lesions, stimulates ATR kinase activity und...
Mdc1 is a large modular phosphoprotein scaffold that maintains signaling and repair complexes at dou...
TopBP1 is a scaffold protein that coordinates activation of the DNA-damage-checkpoint response by co...
SummaryThe tandem BRCT domains of BRCA1 and MDC1 facilitate protein signaling at DNA damage foci thr...
The BRCT-domain protein Rad4(TopBP1) facilitates activation of the DNA damage checkpoint in Schizosa...
SummaryATR is a key regulator of checkpoint responses to incompletely replicated and damaged DNA, bu...
Coordination of the cellular response to DNA damage is organised by multi-domain ‘scaffold’ proteins...
Coordination of the cellular response to DNA damage is organised by multi-domain 'scaffold' proteins...
The cellular response to DNA damage (DDR) that causes replication collapse and/or DNA double strand ...
The activation of ATR-ATRIP in response to double-stranded DNA breaks (DSBs) depends upon ATM in hum...
The cellular response to DNA damage (DDR) that causes replication collapse and/or DNA double strand ...
In mitosis, cells inactivate DNA double-strand break (DSB) repair pathways to preserve genome stabil...
Topoisomerase IIβ binding protein 1 (TopBP1) is a critical protein-protein interaction hub in DNA re...
Human TopBP1 contains nine BRCT domains and functions in DNA replication initiation, checkpoint sign...
In mitosis, cells inactivate DNA double-strand break (DSB) repair pathways to preserve genome stabil...
TopBP1, acting in concert with DNA containing bulky base lesions, stimulates ATR kinase activity und...
Mdc1 is a large modular phosphoprotein scaffold that maintains signaling and repair complexes at dou...
TopBP1 is a scaffold protein that coordinates activation of the DNA-damage-checkpoint response by co...
SummaryThe tandem BRCT domains of BRCA1 and MDC1 facilitate protein signaling at DNA damage foci thr...
The BRCT-domain protein Rad4(TopBP1) facilitates activation of the DNA damage checkpoint in Schizosa...
SummaryATR is a key regulator of checkpoint responses to incompletely replicated and damaged DNA, bu...
Coordination of the cellular response to DNA damage is organised by multi-domain ‘scaffold’ proteins...
Coordination of the cellular response to DNA damage is organised by multi-domain 'scaffold' proteins...
The cellular response to DNA damage (DDR) that causes replication collapse and/or DNA double strand ...
The activation of ATR-ATRIP in response to double-stranded DNA breaks (DSBs) depends upon ATM in hum...
The cellular response to DNA damage (DDR) that causes replication collapse and/or DNA double strand ...
In mitosis, cells inactivate DNA double-strand break (DSB) repair pathways to preserve genome stabil...
Topoisomerase IIβ binding protein 1 (TopBP1) is a critical protein-protein interaction hub in DNA re...
Human TopBP1 contains nine BRCT domains and functions in DNA replication initiation, checkpoint sign...
In mitosis, cells inactivate DNA double-strand break (DSB) repair pathways to preserve genome stabil...
TopBP1, acting in concert with DNA containing bulky base lesions, stimulates ATR kinase activity und...
Mdc1 is a large modular phosphoprotein scaffold that maintains signaling and repair complexes at dou...