Measurement of the acute metabolic response to hypoxia in rat tumours in vivo using magnetic resonance spectroscopy and hyperpolarised pyruvate

Bluff, Joanne E.
Reynolds, Steven
Metcalf, Stephen
Alizadeh, Tooba
Kazan, Samira M.
Bucur, Adriana
Wholey, Emily G.
Bibby, Becky A.S.
Williams, Leigh
Paley, Martyn N.
Tozer, Gillian M.

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Publication date

September 2015

DOI

10.1016/j.radonc.2015.03.011

Publisher

The Authors. Published by Elsevier Ireland Ltd.

ISSN

0167-8140

Citation count (estimate)

Abstract

AbstractPurposeTo estimate the rate constant for pyruvate to lactate conversion in tumours in response to a hypoxic challenge, using hyperpolarised 13C1-pyruvate and magnetic resonance spectroscopy.Methods and materialsHypoxic inspired gas was used to manipulate rat P22 fibrosarcoma oxygen tension (pO2), confirmed by luminescence decay of oxygen-sensitive probes. Hyperpolarised 13C1-pyruvate was injected into the femoral vein of anaesthetised rats and slice-localised 13C magnetic resonance (MR) spectra acquired. Spectral integral versus time curves for pyruvate and lactate were fitted to a precursor-product model to estimate the rate constant for tumour conversion of pyruvate to lactate (kpl). Mean arterial blood pressure (MABP) and oxygen ...